Principle
In the first step, LDL, VLDL & Chylomicrons are eliminated under specific conditions.
Mix and incubate for 5 minutes at 37°C. Measure the absorbance A1 at 578 nm.In the second step, special reagents break up HDL and make HDL Cholesterol available for measurement.
Procedure
Reaction type ………………. End Point (2 Step)
Reaction time ……………….. 5 + 5 mins.
Wavelength ……………………………….. 578 nm.
Flowcell temperature ……………………….. 37°C
Zero setting with ………………………….. Distilled water
Sample volume ……………… 0.01 ml (10 pl)
Reagent volume ……….. ……. 0.750 ml + 0.250 ml
Linearity …. 185 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. Reconstituted calibrator is stable for 3 days at 2° -8°C. Reconstitution stability of the calibrator may be extended upto 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20°C.
Expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range.
Pintu Das –
I am using many biochemistry kits very accurate
Mohan Kale –
Excellent quality product. Cholesterol and Glucose since 4 years. Thank you Accurex
Saili Gudose –
..Accurex providing best quality reagents. cholesterol are Excellent product…
Avinash Pawar –
I m using your products from last 6months..and products have a very good quality.
Rajesh Gupta –
I’m using accurex glucose, cholesterol reagents for my lab product is good, satisfied with the results.