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HDL – Cholesterol Direct 20 – Infinite
₹2,309.00
Overview
Buy Infinite Liquid HDL Cholesterol Direct 20 Diagnostic Reagent from Accurex (R1 X 15ml: R2 1X 5ml: Calibrator: 1 X 1ml). HDL Cholesterol Direct Reagent is used for the determination of HDL-Cholesterol in human serum or plasma based on the direct homogeneous method. Direct measurement by Infinite HDL-C overcomes the limitation of the precipitation method, thereby allowing accurate determination of HDL–C. It is linear upto 185 mg/dl & is a ready-to-use: two liquid reagent system stable till expiry.
About
1. Infinite HDL-C is a reagent set for direct quantitative determination of HDL-Cholesterol in human
2. serum or plasma based on the direct homogeneous method.
3. Direct measurement by Infinite HDL-C overcomes the limitation of the precipitation method, thereby allowing accurate determination of HDL-C.
4. Infinite HDL-C is a ready-to-use, two-liquid reagent system stable till expiry.
5. Infinite HDL-C is linear up to 185 mg/dl.
6. There is no significant interference in hyperlipemic and hyperimmunoglobulin samples
7. Results obtained with Infinite HDL-C show excellent correlation with ultracentrifugation methods.
Principle
In the first step, LDL, VLDL & Chylomicrons are eliminated under specific conditions.
Mix and incubate for 5 minutes at 37°C. Measure the absorbance A1 at 578 nm. In the second step, special reagents break up HDL and make HDL Cholesterol available for measurement.
Quality Control
To ensure adequate quality control, it is recommended that each batch include a normal and an abnormal commercial reference control serum. It should be realised that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Direction for Use
Reaction type ………………. End Point (2 Step)
Reaction time ……………….. 5 + 5 mins.
Wavelength ……………………………….. 578 nm.
Flowcell temperature ……………………….. 37°C
Zero setting with ………………………….. Distilled water
Sample volume ……………… 0.01 ml (10 pl)
Reagent volume ……….. ……. 0.750 ml + 0.250 ml
Linearity …. 185 mg/dl
Other Info
The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. Reconstituted calibrator is stable for 3 days at 2° -8°C. Reconstitution stability of the calibrator may be extended upto 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20°C.
The expected range varies from population to population. It is therefore recommended that each laboratory should establish its own normal range.
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