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GOT (AST) 50
₹1,071.00
Overview
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Infinite Liquid GOT (AST) 50 Diagnostic Reagent
(R1 1 X 40ml: R2: 1 X 10ml) from Accurex. Glutamate Oxaloacetate Transaminase reagent is used for determination of True Glucose, based on enzymatic method using Glucose, oxidase & Peroxidase.
Accurex Infinite Liquid GOT (AST) can be used on any Spectrophotometer, Discrete semi automated & Automated analyzers. Programme can be designed for any specific analyzer upon request.
50 in stock
About
- Infinite GOT (AST) 100 (Liquid) is a reagent set for determination of GOT (AST) activity in serum and plasma based on UV – Kinetic method.
- Infinite Liquid GOT (AST) is a ready-to-use, two liquid reagent system.
- Infinite Liquid GOT (AST) estimates GOT (AST) activity in just 2.5 minutes.
- Infinite Liquid GOT (AST) is linear up to 800 IU/.
- Infinite Liquid GOT (AST) can be used on any Spectrophotometer, Discrete semi automated and Automated analyzers. Programme can be designed for any specific analyzer upon request.
- Infinite Liquid GOT (AST) is stable till expiry at 2 – 8°C.
Method – IFCC
Principle
α-ketoglutarate reacts with L-aspartate in presence of GOT (ASTI to form Oxaloacetate and L-glutamate. The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenase. The conversion of NADH to NAD at 340 nm. is proportional to the activity of GOT (AST) in serum/plasma and is determined kinetically as rate of decrease in absorbance.
Procedure
Reaction type ……… UV – Kinetic
Reaction direction ….. Decreasing
Wavelength……………. 340 nm.
Flow Cell temperature ………………… 37°C
Zero setting with . Distilled water
Delay time …. 60 seconds
No. of readings .. ……..4
Interval …. 30 seconds
Blank absorbance limit ………. ….. 0.900 Abs.
Sample volume ……………. 0.05 ml (50 ul)
Working solution volume (4 R,:1 R.)………….. 1.0 ml (1000 jul)
Factor ……………….. O
Linearity ………….. 800 IU/
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label.
R, and R, reagents are stable till expiry at 2-8°C.
The working solution (4 R. +1R) is stable for 30 days at 2-8°C..
1 review for GOT (AST) 50
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Avnish Gupta –
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