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Diluent Uric Acid 450
₹1,500.00
Overview
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Autozyme Diluent Uric Acid 465 ml Diagnostic Reagent
from Accurex. Uric Acid reagent is used for determination of Uric Acid based on enzymatic method using Uricase and Peroxidase. Autozyme Uric Acid is a single reagent system & can be determined in 8 minutes.
Certain foods filled with dietary fibres are extremely beneficial in the uric acid treatment & in eliminating Uric Acid in body. Gout is a form of inflammatory arthritis which is very painful. Uric acid Reagent is stable for 3 days at room temperature (below 25°C) and for 6 months when stored at -10°C.
50 in stock
About
- AutoZyme NEW Uric Acid is a reagent set for determination of Uric Acid based on enzymatic methods using Uricase and PerOxidase.
- AutoZyme NEW Uric Acid is a single reagent system.
- AutoZyme NEW Uric Acid is linear up to 25 mg%.
- AutoZyme NEW Uric Acid is a High Stability Reagent.
- AutoZyme NEW Uric Acid can be used on any Colorimeter Spectrophotometer, Discrete semi automated and Automated HEPARIN analyzer. Programme can be designed for any specific analyzer upon request.
- Uric Acid assay can be performed in 10 minutes SODIUM I The influence of Glucose, Bilirubin, Ascorbate, Allopurinol, Urea, Protein, EDTA, Fluoride, citrate, Oxalate and Haemoglobin is
negligible. - The shelf-life of Autozyme NEW Uric Acid is 15 months.
Principle
Uricase converts uric acid into allantoin and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidatively couples with phenolic chromogens to form a red coloured compound, which has maximum absorbance at 510 nm. (500-530 nm.). The concentration of the red coloured compound is proportional to the amount of uric acid in specimens.
Procedure
Reaction type ………….. End Point
Reaction time…………….10 mins. at R.T. (25 – 30°C)
Wavelength…………..510 nm. (500-530 nm.)
Zero setting with……….Reagent Blank
Blank absorbance limit……………<0.300 Abs.
Sample volume…………….0.025 ml (25 l)
Reagent volume…………….1.0 ml
Standard concentration…………..6 mg%
Linearity…………….25 mg
Manual assay procedure
Prewarm at room temperature the required amount of Working reagent before use.
Perform the assay as given below: 1.0 ml procedure
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of the test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
- The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label.
- The working reagent should be stored always at 2- 8°C
Stability of the reconstituted working reagent is printed on the bottle label.
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Sivam Malvia –
Company product is very good