Principle
Glucose oxidase (GOD) converts Glucose to gluconic acid. Hydrogen
peroxide formed in this reaction, in presence of Peroxidase (POD),
oxidatively couples with 4-aminoantipyrine / phenol to produce red
quinoneimine dye. This dye has absorbance maximum at 505 nm.
(500-550 nm.). The intensity of the color complex is directly proportional to the Glucose in the specimen.
Procedure
Reaction type…..2 Point-Kinetic
Reaction direction………………Upward
Wavelength…………..505 nm. (500- 550 nm.)
Flow cell temperature ……37°C
Zero setting with……Reagent Blank
Delay time…………………………..20 secs
No. of readings…………………..2
Interval……………………………………..40 secs.
Blank absorbance limit……………..< 0.300 Abs.
Sample volume…………0.01 ml (10)
Reagent volume……….1.0 ml
Factor……………..100 / (A Abs. of std.)
Linearity…………………..700 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of the test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
Stability of the reconstituted working reagent is printed on the bottle label. (DO NOT FREEZE). The working solution should be stored in the dark bottle (diluent bottle or the working solution bottle) provided. This is critical because the reagent is light sensitive (auto oxidation of chromogen system by light and air), it should therefore be kept away from direct light.
Sunil Padwal –
Good quality and also good MRP