Principle
Calcium forms a purple color complex with cresol phthalein complexone in alkaline medium. This complex absorbs light at 575 (570 – 580) nm. The intensity of the color is directly proportional to the calcium concentration in the specimen.
Procedure
Reaction type………………. End-Point
Reaction time………………….. 5 mins. at R.T. (25-30°C)
Wavelength…………………… 575 nm.(570 – 58 aim.)
Zero setting with ……………………… Reagent Blank
Sample volume……………………. 0.02 ml (20 11 1 )
Reagent volume…………………………….. 1.0 ml
Standard concentration ……………….10 mg%
Linearity…………………………… 15 mg/di
Quality Control
To ensure adequate quality control, it is recommended that each batch should include normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of Pipetting.
Jayesh Singh –
We are using accurex regents more than 8 parameters since 4 year’s the product quality is best
Mannoj Yadav –
I’m fully satisfied with this reagent quality and services