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Blood Grouping Antisera
Overview
Accurex Anti A, Anti B, and Anti D kit contains test reagents for the determination of antigens of the ABO system including the determination of subgroups of antigen A.
Buy Accurex Anti A, Anti B, and Anti D antisera for Accurate detection of BLOOD GROUPS of HUMAN available in the pack size of 3 x 10 ml in sealed bottles.
About
Intended use for Test reagents for determination of antigens of the ABO system, including determination of subgroups of antigen A
Product Specifications
| Method | Monoclonal |
| Technique | Agglutination |
| Detection | Antigens of ABO and Rh sytem |
| Class of antibody | |
| Titre | Anti A and Anti B- 1:512 Macroscopically Anti D- 1:256 Macroscopically |
| Avidity | <10 sec |
Safety Information
- Although Monoclonal sera contains preservatives, care should be taken to avoid microbial contamination.
- Do not interchange caps of vials and avoid the use of turbid reagents.
- Bring reagents and samples to room temperature before use.
- Suppressed or diminished expression of certain blood group antigens may conversely give rise to false negative reactions.
- Do not interpret peripheral drying or fibrin strands as and handle with due care at all times during testing and Disposal.
Direction for Use
TUBE TEST:
The reagent has been characterized by the recommended procedure. Before release, each of lot Anti A, B, and AB reagents is tested by the recommended procedures against a panel of antigen-positive red cells to ensure suitable reactivity.
The specificity of source monoclonal antibodies is shown using a panel of antigen-negative cells. Anti-B does not react with “Acquired-B” red cells. Monoclonal ABO reagents do not detect crypt antigens such as T, Tn, or Cad.
- Place one drop of Anti-A, Anti-B, or Anti-AB on clean and dry slide.
- Add one drop of whole blood or 40% RBC’s suspension prepared in the individual’s own serum
- Mix well with an applicator stick; gently tilt the slide forward and backward at room temperature for a maximum of 2 minutes.
Tube Test:
- Use an 8×50 mm small glass test tube for each specimen, take a tube and label it with the name or code number of the patient
- Prepare 5% suspension of the RBCs to be tested in isotonic saline.
- Add one drop of Anti-A, or Anti-B, or Anti-AB reagent and saline to the respective tubes
- Add one drop of cell suspension to each tube and mix well.
- Shake each tube thoroughly and centrifuge for 1 minute at 1000 RPM (125g)
Other Details
Ordering information
| Material Description | Pack size |
| Anti A | 1 x 10 ml |
| Anti B | 1 x 10 ml |
| Anti AB | 1 x 10 ml |
| Anti D | 1 x 10 ml |
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