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Autopure-RF (4:1)
₹5,800.00
Overview
The RF-Turbilatex is a quantitative turbidimetric test for the Measurement of RF in human serum or plasma. Latex particles coated with human gamma-globulin are agglutinated when mixed with samples containing RF. The agglutination causes an absorbance change, dependent upon the RF contents of the sample, that can be quantified by comparison to a calibrator of known RF concentration.
About
The RF-Turbilatex is a quantitative turbidimetric test for the Measurement of RF in human serum or plasma. Latex particles coated with human gamma-globulin are agglutinated when mixed with samples containing RF. The agglutination causes an absorbance change, dependent upon the RF contents of the sample, that can be quantified by comparison to a calibrator of known RF concentration
Specification
1. Limit detection: Values less than 6 IU/mL give non-reproducible results.
2. Measurement range: 6-160 IU/mL, under the described assay
conditions. Samples with higher concentrations should be diluted
1/5 in NaCl 9 g/L and retested again. The linearity limit and
measurement range depend on the sample-to-reagent/ratio and the analyzer used. It will be higher by decreasing the
sample volume, although the sensitivity of the test will be
proportionally decreased.
3. Prozone effect: No prozone effect was detected at 800 IU/mL.
4. Sensitivity: 3,34 mA. IU/mL.
5. Precision: The reagent has been tested for 20 days, using
three different FR concentrations in an EP5-based study.
Safety Information
PRECAUTIONS: Components from human origin have been tested and found to be negative for the presence of HBsAg, HCV, and antibody to HIV (1/2). However, handle cautiously as potentially infectious
Direction for Use
CALIBRATION
Use RF Calibrator Reference 1107007. The sensitivity of the assay and the target value of the calibrator have been standardized against the International Reference NIBSC 64/2 (Rheumatoid Arthritis Serum) WHO.
Calibration Curve: Prepare the following RF calibrator dilutions in NaCl 9 g/L. Multiply the concentration of the RF calibrator by the corresponding factor stated in the table below to obtain the RF concentration of each dilution.
Ready for use
RF Calibrator: Reconstitute () with 2,0 mL of distilled water. Mix gently and bring to room temperature for about 10 minutes before use.
For more details, refer Pack insert.
Other Info
SAMPLES
Fresh serum or plasma. Stable 7 days at 2-8ºC or 3 months at –20ºC. The samples with the presence of fibrin should be centrifuged before testing. Do not use highly hemolyzed or lipemic samples.
REFERENCE VALUES
Normal values up to 20 IU/mL. Each laboratory should establish its own reference range.
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