Principle
The chromogenic Lipase substrate 1,2 – 0 – dilauryl| – rac – glycero – 3 glutaric acid-(6-methyl resorufin) ester is cleaved by the catalytic action of lipase to form 1,2 – 0 – dilauryl-rac- glycerol and an unstable intermediate glutaric acid -(6 – methyl resorufin) ester. This decomposes spontaneously in alkaline medium to form glutaric acid and methyl resorufin.
Procedure
AutoPure Lipase can be used on various automated analyzers.
Quality Control
Each batch of AutoPure Lipase is assayed with at least six quality control sera — Precinorm**, Precipath**, Biorad l***, Biorad II***, Sero Low**** and Sero High****prior to release. To ensure adequate quality control, it is recommended that the laboratory should use a normal and an abnormal commercial reference control serum.
It should be realized that the use of quality control material checks both reagent and instrument functions together. The value of these controls should fall within the specified limits. If control values fall outside specified limits, each of the below criteria should be cross-checked and corrected :
• Proper instrument function – wavelength setting, light source and temperature control
• Cleanliness of probes & cuvettes
• Bacterial contamination of wash water used by the instrument
• Expiry date of the reagent kit
Harsha Bagat –
Lipase 30 Reagent is used for direct quantitative determination of Lipase using with Accurex Spera automated clinical chemistry analyzers.
Prasad –
AutoPure Lipase 30 seems like a good option for measuring lipase in human serum and plasma. 24