Principle
Lactate dehydrogenase catalyzes the conversion of pyruvate to lactate.
The conversion of the NADH to NAD is directly proportional to the LDH activity in the specimen is measured kinetically.
Procedure
AutoPure LDL-C can be used on various automated analyzers.
Quality Control
Each batch of AutoPure GPT (ALT) is assayed with at least six quality control sera — Precinorm**, Precipath**, Biorad l***, Biorad II***, Sero Low**** and Sero High****prior to release.
To ensure adequate quality control, it is recommended that the laboratory should use a normal and an abnormal commercial reference control serum.
It should be realized that the use of quality control material checks both reagent and instrument functions together. The value of these controls should fall within the specified limits. If control values fall outside specified limits, each of the below criteria should be cross-checked and corrected :
• Proper instrument function – wavelength setting, light source and
temperature control
• Cleanliness of probes & cuvettes
• Bacterial contamination of wash water used by the instrument
• Expiry date of the reagent kit
Ishaan Niral –
Quality reagent with linear upto 1200 IU
Subodh –
Rapid results are obtained using these product