Principle
α-ketoglutarate reacts with L-aspartate in the presence of GPT (ALT) to form oxaloacetate and L-glutamate.
The increase in oxaloacetate is determined in an indicator reaction catalyzed by malate dehydrogenase.
The conversion of NADH to NAD+ is proportional to the GPT (ALT) activity in the specimen and is determined kinetically.
Procedure
AutoPure GPT (ALT) can be used on various automated analyzers.
Quality Control
Each batch of AutoPure GPT (ALT) is assayed with at least six quality control sera — Precinorm**, Precipath**, Biorad l***, Biorad II***, Sero Low**** and Sero High****prior to release.
To ensure adequate quality control, it is recommended that the laboratory should use a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both reagent and instrument functions together. The value of these controls should fall within the specified limits. If control values fall outside specified limits,
each of the below criteria should be cross-checked and corrected :
• Proper instrument function – wavelength setting, light source and
temperature control
• Cleanliness of probes & cuvettes
• Bacterial contamination of wash water used by the instrument
• Expiry date of the reagent kit
Madhur –
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