Principle
Serum albumin in the presence of Bromocresol-green under acidic condition
forms a green coloured complex, The absorbance of this complex is proportional to the albumin concentration in serum or plasma.
Procedure
Reaction type ………………. End-Point
Reaction time ……………………..1 min. at R.T. (25-30°C)
Wavelength ………………………… 600 nm.(600 – 650 nm.)
Zero setting with …………………. Reagent Blank
Blank absorbance limit ……………….. < 0.100 Abs.
Sample volume …………….0.01 ml (10 ul)
Reagent volume …………………….. 1.0 ml
Standard concentration ……………. 5 gm%
Linearity. ………….. 6 gm%
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum,. it should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagents are for in vitro diagnostic use.
BCG reagent and standard should be stored at temperature indicated on the bottle label.
Viswanath Kale –
I am using accurex reagents. Very good and satisfied. I am very happy with accurex biomedicals
Keval –
The reagent performs well in terms of accuracy and ease of use. Rapid results are obtained using these product
Kiran –
Using Albumin 300 from last 1 year, Product is good for use. Perfect for Healthcare