About
INTENDED USE
AccuTest Vitamin D is a rapid chromatographic immunoassay for the semi quantitative detection of Vitamin D levels in human blood/serum. This assay provides only a preliminary screening test result. A more specific alternative chemical method must be used in order to obtain a confirmed analytical result. The performance characteristics of this assay have not been established in a pediatric population.
Specification
PRINCIPLE
AccuTest Vitamin D is based on the principle of a competitive enzyme immunoassay. The assay is based on the competition for 25-OH Vitamin D present in blood/serum sample and Vitamin D present on the test line for fixed number of antibody-gold conjugate. Depending upon the concentration of Vitamin D in blood/serum, there will be free antibody-gold conjugate
molecules that will bind to Vitamin D on the test strip and will show a colored line in the test line zone. A control line is present in the test window to work as procedural control.
Direction for Use
PROCEDURE
1. Remove the cassette from the sealed pouch and place it on a hard flat surface with the view window facing up (use the cassette as soon as possible).
2. Using a micropipette, add 10 µl of whole blood or 5ul of serum sample directly into the rectangular specimen well (marked with Red drop) of the cassette.
3. Add 3 full drops of Buffer into the square buffer well of the cassette (Do not move the cassette after addition of buffer).
4. Let the cassette sit for 10 minutes and read your results immediately. Results may deteriorate after the 10 minute mark.
Other Info
STORAGE AND STABILITY
The test device should be stored at 8 ⁰C – 30 ⁰C and will be stable until the expiration date stated on the package. The product is humidity sensitive and should be used immediately after being open.
INTERPRETATION OF RESULTS
Match the color of the test line with the chart provided.
Rajan Bhise –
Accurate and reliable testing
Shankar Kumar –
Best Rapid test kit
Raj Sharma –
Accurate result. Must buy
Vaneeta –
Accurate and convenient to use
Sunil Narayan –
Satisfied result and very good quality.