About
The Renal Function Test Strip use a timed-endpoint method to measure the Uric Acid (UA),Creatinine (CR) and Urea (UR) concentrations in whole blood, serum or plasma. The system monitors the change in absorbance at 620 nm at a fixed-time interval. The change in absorbance is directly proportional to the concentration of metabolite in the specimen.
UA: In the reaction, the uric acid is oxidized to allantoin and hydrogen peroxide by uricase.
Peroxidase catalyzes the reaction of hydrogen peroxide with 4-aminoantipyrine and phenol to produce a colored quinonimine product. The change in absorbance is directly proportional to the
concentration of uric acid in the specimen.
CR: Creatinine in the specimen are hydrolyzed to creatine by the action of creatinine. A sequence
of three coupled enzymatic steps using keratinase, sarcosine oxidase, and peroxidase causes the oxidative coupling of 4-aminoantipyrine to form a blue dye.
UR: Urea in the specimen are hydrolyzed to NH3 and CO2 by the action of urease and an indicator layer containing an indicator which produces a detectable change by gaseous ammonia.
Direction for Use
Allow the test strip, specimen, and/or controls to reach operating temperature (10-35℃) prior to
testing.
Refer to the Renal Function Monitoring System or Dry Biochemical Monitoring System User’s Manual or for detailed instructions.
1. Insert the code chip into the meter and code the meter correctly. Refer to Coding the meter section in the User’s Manual for details. Compare the code number on the code chip with the code number printed on the test strip canister or foil pouch and ensure the two number are identical to avoid inaccurate results.
2. Check that the specimen type displayed on the meter LCD is same as the specimen type tested. If not, set the correct specimen type. Refer to the User’s Manual for details.
3. Remove the test strip from the test strip canister or foil pouch.
4. Wait for the meter to flash the test strip symbol. Insert the test strip completely into the test strip channel in the same direction as the arrows printed on the test strip.
5. Prepare the specimen to be tested. For venous whole blood/plasma/serum specimens: mix the specimen for about 15minutes. For capillary blood specimen: wipe away the first drop of
blood. Collect 35μL of the second or third drop of capillary blood specimen using a Capillary Transfer Tube or pipette. Refer to the User’s Manual for details. Hold the tube slightly downward and touch the tip of the Capillary Transfer Tube to the blood drop.
Draw the specimen and stop drawing when the specimen comes to the fill line.
6. While the meter is flashing the blood drop symbol, apply 35μL specimen to the Specimen Application Area of the test strip using a pipette or Capillary Transfer Tube. Align the tip of
he pipette or capillary Transfer Tube with the Specimen Application Area to apply the blood. 4 dashed lines will appear on the meter to show the test is in progress.
7. Read the results on the screen in 5 minutes. Refer to the User’s Manual for detail test procedures.
Note: Use safety lancets for tests. Avoid an environment with strong lighting during the test. Be sure the alcohol dries completely before picking the finger. Hand lotions or creams on the finger
should be cleaned enough before testing. Excessively squeezing the finger may alter the results. Add 35μL specimen to the test strip at one time.
Other Info
Store as packaged in the sealed pouch or canister at room temperature or refrigerated (2-30℃).
Keep out of direct sunlight. Test strips are stable through the expiration date printed on the test strips canister or foil pouch.
Remove only enough test strips for immediate use. Replace the cap on the strips canister immediately and tightly.
DO NOT FREEZE. Do not use beyond the expiration date.
Krishnakant –
Accurate result for urea, creatinine