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Urea 200
₹1,926.00
Overview
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Autozyme Urea 200 Diagnostic Reagent
from Accurex of {Pack Size : 2 x 100 det. (400 ml)}. Urea reagent is used for determination of Urea/Blood Urea Nitrogen based on enzymatic method using Urease.
Urea 200 is a two reagent system using two step procedure with linear up to 350 mg% & determined in 8 minutes. chromogen solution is stable for 6months at 2-8°C after opening. 6 to 24 mg/dL (2.1 to 8.5 mmol/L ) is considered as urea normal range.
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About
-Autozyme Urea is a reagent set for determination of Urea/Blood Urea Nitrogen based on enzymatic method using Urease
-Urea can be determined in 8 minutes
-Autozyme Urea is linear up to 350 mg%
– Autozyme Urea is a two reagent system using two step procedure
-The shelf life of Autozyme Urea is 18 months
Pack Size : 2 x 50 det. (200 ml)
Principle
Urease splits urea into ammonia and carbon dioxide. Ammonia released in this reaction reacts with hypochlorite and phenolic chromogen to produce green color. The absorbance of this green color at 578 nm. (570-620) is directly proportional to the concentration of urea in specimens.
Procedure
Reaction time …………………………. 3+ 5 min.
Wavelength …….. 578 nm. (570-620 nm.)
Zero setting with. …….. Reagent Blank
Blank absorbance limit ………… <0.200 Abs.
Sample volume….. ……. 0.01 ml (10)
Reagent volume ……………………. 1.0 ml + 1.0 ml
Standard concentration………….. 40 mg%
Linearity ……….350 mg/dl
Quality Control
To ensure adequate quality control, it is recommended that each batch should include a normal and an abnormal commercial reference control serum. It should be realized that the use of quality control material checks both instrument and reagent functions together. Factors which might affect the performance of this test include proper instrument function, temperature control, cleanliness of glassware and accuracy of pipetting.
Other Details
The reagent kit should be stored at 2-8°C and is stable till the expiry date indicated on the label. The reconstituted enzyme solution is stable for 6 months when stored at 2-8°C. DO NOT FREEZE THE ENZYME SOLUTION. The chromogen solution is stable for 6months at 2-8°C after opening.
6 reviews for Urea 200
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